Performance Evaluation Of The Clinimacs System For The Positive Selection Of CD34+ Cells From Stem Cell Products Factors Affecting CD34+ Cell Recovery
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- Año de publicación 2009
- Idioma Inglés
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- CD34+ cell dose in the transplanted product is still considered a measure of the engraftment potential in haemopoietic stem cell transplantation (HSCT). CD34+ selected products are characterised by their high purity and depleted T-cells. These characteristics have made them traditionally suitable to decrease the incidence of graft versus host disease, particularly in haploidentical HSCT.The CliniMACS is a state of the art cell sorting system based on magnetic particles conjugated to CD34 antibodies. This study retrospectively evaluated the CliniMACS system performance in terms of CD34+ purity and cell recovery, and T-cell depletion in the selected product. The results were compared against target values fixed by the processing centre, as well as against published data. Factors affecting the CD34+ cell recovery were also investigated and a pilot study was carried out to assess the effect of cryopreservation on the viability of the selected products. In total, 151 procedures were analysed.The mean recovery and purity were 64.82 + 16.19 and 88.0 + 11.0% respectively. The mean T-cell depletion was 4.7021 + 0.5129 logs. Only purity was slightly below the specification (90%), with the lowest values obtained from peripheral blood (PB) collections. Factors affecting the recoveries were the overall age of the product (p=0.001), overnight storage (p=0.002), and the use of a particular tubing set with the instrument (p=0.002).The mean CD34+ viability was not significantly different to the value before cryopreservation (p=0.068). Results from this study were comparable with published data of the CliniMACS system performance. Local investigations regarding the purity of PB products should be performed. For the first time, using a magnetic separation method, factors affecting CD34+ recovery were identified. The significance of the effect of product age and overnight storage on cell recovery, provide a rationale for further prospective studies
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Citación recomendada (normas APA)
- Guillermo Andrés Orjuela Falla, "Performance Evaluation Of The Clinimacs System For The Positive Selection Of CD34+ Cells From Stem Cell Products Factors Affecting CD34+ Cell Recovery", -:-, 2009. Consultado en línea en la Biblioteca Digital de Bogotá (https://www.bibliotecadigitaldebogota.gov.co/resources/2084163/), el día 2024-05-03.
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