Tesis

Investigation of the molecular evolution of carbapenem resistance in Klebsiella quasipneumoniae

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Año de publicación 2021
Idioma Inglés

Descripción: Abstract: The evolution of multidrug resistance in Klebsiella species in response to selective pressure and the extent to which this may be reversible is not fully understood. The spread of carbapenemresistant Enterobacteriaceae (CRE) is considered a public health threat by the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC). As such, more studies are needed to understand the evolution of antibiotic resistance mechanisms to determine if it is possible to reverse this trend. Highlighted In this study, a comprehensive characterisation of a clinical Klebsiella quasipneumoniae isolate called FK688 is performed. The FK688 strain is resistant to multiple antibiotics, including third-generation cephalosporins and carbapenems and caused a bloodstream infection in a hospitalised patient. A combinational approach of genomic, enzymatic, and machine learning analyses did not uncover any carbapenemase-encoding genes in FK688. Rather, this research showed that epistatic changes are necessary for FK688 to acquire a CRE phenotype. Evolution experiments demonstrated the fitness burden associated with antimicrobial resistance determinants and the reversion to a carbapenem-susceptible phenotype in an antibiotic-free environment. Fitness assays showed that a low concentration of ceftazidime selects for a ?- lactamase gene and can potentiate evolution to carbapenem resistance by a single-step mutation in the porin OmpK36. This study demonstrated the importance of epistatic events and how variation in drug exposure can shape the evolutionary pathway to antibiotic resistance in bacteria.
Citación recomendada (normas APA)
: Natalia Carolina Rosas Bastidas, "Investigation of the molecular evolution of carbapenem resistance in Klebsiella quasipneumoniae", Colombia:-, 2021. Consultado en línea en la Biblioteca Digital de Bogotá (https://www.bibliotecadigitaldebogota.gov.co/resources/3711797/), el día 2025-08-21.

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- Otros
- Medicina
Derechos de uso Derechos reservados
Fuente Biblioteca Virtual Banco de la República
Términos y condiciones de uso Más información aquí
Año de publicación 2021
Formato PDF
Cobertura Colombia

Investigation of the molecular evolution of carbapenem resistance in Klebsiella quasipneumoniae

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Investigation of the molecular evolution of carbapenem resistance in Klebsiella quasipneumoniae

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