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Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT)

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Año de publicación 2019
Idioma Inglés
Publicado por Scientific Reports / https://www.nature.com/srep/ Scientific Reports is an online multidisciplinary, open access journal from the publishers of Nature

Descripción: Organotypic brain culture is an experimental tool widely used in neuroscience studies. One major drawback of this technique is reduced neuronal survival across time, which is likely exacerbated by the loss of blood flow. We have designed a novel, tube flow system, which is easily incorporated into the commonly-used, standard semi-permeable membrane culture methodology which has significantly enhanced neuronal survival in a brain stem nucleus involved in control of motivated and arousal states: the laterodorsal tegmental nucleus (LDT). Our automated system provides nutrients and removes waste in a comparatively aseptic environment, while preserving temperature, and oxygen levels. Using immunohistochemistry and electrophysiology, our system was found superior to standard techniques in preserving tissue quality and survival of LDT cells for up to 2 weeks. In summary, we provide evidence for the first time that the LDT can be preserved in organotypic slice culture, and further, our technical improvements of adding a flow system, which likely enhanced perfusion to the slice, were associated with enhanced neuronal survival. Our perfusion system is expected to facilitate organotypic experiments focused on chronic stimulations and multielectrode recordings in the LDT, as well as enhance neuronal survival in slice cultures originating from other brain regions.
Citación recomendada (normas APA)
: César R.; Elnagar Romero Leguizamón, "Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT)", Colombia:Scientific Reports / https://www.nature.com/srep/ Scientific Reports is an online multidisciplinary, open access journal from the publishers of Nature, 2019. Consultado en línea en la Biblioteca Digital de Bogotá (https://www.bibliotecadigitaldebogota.gov.co/resources/3711169/), el día 2025-05-04.

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- Medicina
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Fuente Biblioteca Virtual Banco de la República
Términos y condiciones de uso Más información aquí
Año de publicación 2019
Formato PDF
Cobertura Colombia

Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT)

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Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT)

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