Abstract: This thesis analyses the Colombian government’s decision-making process that involved international actors during the Havana peace process (2012-2016). The research highlights the influence of international actors in the government’s decisions that worked to end the armed conflict between the government and the FARC-EP in Colombia. In particular, this study raises the following research question: How, and in what way, was the Colombian government’s decision-making process influenced by international actors, especially the presence and actions of the UN, during the Havana peace process? The hypothesis of this research expects that the decision-making of the Colombian government during the Havana peace process had a key political condition of clearly defined involvement from international actors. It focuses on the Colombian Executive branch as the main actor and its relations with other national and international actors. To do so, the research references the theories of Foreign Policy Analysis, including the Governmental Politics Model and the Two-Level Game Model. This thesis provides an in-depth analysis of the Colombian government’s decision-making process that involved UN cooperation by applying an interpretative case study. The research methodology is built on data collection gathered by interviewing a number of high-profile individuals involved in the Havana peace process including members of the Colombian government, members of the former guerrilla FARC-EP, United Nations Officials, and national and international academics and advisors who were involved in the decision-making during the negotiations. The conclusion goes on to argue that the Colombian decision-making process implemented during the negotiations was designed precisely to effectively define the role of international support, which improved the likelihood of success of the final peace agreement. The cooperation of international actors, especially from the UN, built trust between the negotiating parties regarding the sustainability of the negotiations. In addition, the international cooperation promoted institutional and political conditions that were necessary to achieve the final peace agreement. Resumen: Esta tesis analiza el proceso de toma de decisiones del gobierno colombiano que incluyó la participación de actores internacionales en el proceso de paz de La Habana (2012-2016). La investigación destaca la influencia de los actores internacionales en las decisiones gubernamentales que facilitaron la terminación del conflicto armado entre el gobierno y las FARC-EP en Colombia. Particularmente, este estudio responde a la siguiente pregunta de investigación: ¿Cómo y de qué manera el proceso de toma de decisiones del gobierno colombiano fue influenciado por actores internacionales, especialmente la presencia y acción de la ONU durante el proceso de paz de La Habana? La hipótesis de esta investigación argumenta que el proceso de toma de decisiones del gobierno colombiano durante el proceso de paz de La Habana requirió una condición política de tener claramente definidos los límites de la participación de actores internacionales. La investigación enfoca en la rama de poder Ejecutivo colombiano como actor principal y en sus relaciones con otros actores nacionales e internacionales. Para esto, la investigación se basa en teorías de Análisis de Política Exterior, incluyendo el Modelo de Política Gubernamental y el Modelo del Juego de Dos Niveles. Esta tesis proporciona un profundo análisis del proceso de toma de decisiones del gobierno colombiano que incorporó la cooperación de la ONU, aplicando un estudio de caso interpretativo. La metodología de investigación se fundamenta en la recolección de datos, entrevistando varios individuos de alto nivel involucrados en el proceso de paz colombiano, incluyendo miembros del Gobierno colombiano, miembros de la ex guerrilla de las FARC-EP, funcionarios de las Naciones Unidas, académicos y asesores nacionales e internacionales. La conclusión de la investigación argumenta entonces que el proceso de toma de decisiones colombiano implementado durante las negociaciones se organizó de manera precisa para definir efectivamente el papel del apoyo internacional, que aumentaría la probabilidad de éxito para un acuerdo final de paz. La cooperación de actores internacionales, especialmente de la ONU, creó confianza entre las partes negociadoras en favor de la sostenibilidad de las negociaciones. Adicionalmente, la cooperación internacional promovió condiciones institucionales y políticas que fueron necesarias para alcanzar el acuerdo final de paz.

Abtract: Small-scale farming systems in the Colombian Andes are vulnerable to threats that affect not only the food production and self-sufficiency of local communities, but that can affect the wellbeing of farmers and the environment. A holistic perspective of agriculture leads to understanding of the complexity of agroecosystems including its elements, processes, dynamics, interactions, synergies and trade-offs. This thesis examines the main characteristics, contexts and enabling environment for small-scale farming systems in Guachetá, Colombia, in order to identify and analyse the main agricultural problems in the municipality and possible solutions for them. Using the Tool for Agroecology Performance Evaluation (TAPE), the 10 elements of agroecology were evaluated on 7 farms along with the assessment of the performance of the systems using core performance criteria based on 5 key dimensions of sustainability. Furthermore, the analysis of qualitative data obtained from interviews with farmers and other actors, such as associations’representatives and academics in the fields of soils and agricultural science, facilitated the understanding of their perspectives in relation to the challenges that peasant farmers face. The results of this study show that the prevalence of dry climatic conditions and the variation on the typical rain patterns pose a major challenge for the current production system. In addition, the enabling environment, in regards to responsible governance and circular and solidarity economy, constitutes one of the major limitations. Currently, there are negligible possibilities for participation on the governance of the land and there is limited access to markets that offer fair conditions and proximity between consumers and producers. Also, the current agricultural practices reflect dependency on agrochemical inputs that, according to the analysis of the elements of agroecology, leads to low efficiency of the systems, limited agricultural biodiversity and low synergies within the agroecosystems. It is concluded that implementation of agroecological principles and practices that resemble natural ecological processes and that are characterized by joint action can aid in overcoming these issues leading to the improvement of the sustainability of food systems in Guachetá.

Abstract: Latin American countries are experiencing a significant increase in the reports of mistreatment that women experience while using the maternity healthcare services, a phenomenon acknowledged as “Obstetric Violence” (OV). The Latin American region is crucial for the recognition of OV, not only because the term OV was originated there, but also because of its particular response to the matter. The present Scoping Review aimed to identify and review the available evidence about the response of Latin American countries to OV. As a result, it was found, firstly, that the emergence of the concept of OV in Latin America demonstrated to be a transformative tool useful to raise awareness about the mistreatment women face when giving birth. This OV concept also positions this problematic as subset of gendered violence and as a type of structural violence, through which the need to address it systemically is highlighted. Secondly, the used categorization of OV appears to be the broadest and more inclusive typology, emphasizing that OV might stem from both intentional and unintentional actions of healthcare professionals, as well as from conditions within healthcare organizations. Moreover, the identified variables that influence the response to OV in Latin America were synthetized into a theoretical framework, which offers insight into how the problem has been addressed until now and subsequently, provides useful lessons for other countries seeking approaches to combat mistreatment of women during childbirth. Lastly, it was determined that, in order to address OV and allow the progression of public policies to become regionally available, a broadened view encompassing a human rights-based approach is necessary. This research allowed to conclude that OV is a multi-faceted and complex phenomenon whose solution requires a multidimensional and multidisciplinary approach. Furthermore, OV should be understood as more than a simple act of mistreatment, but rather be viewed as a set of socially constructed symbolic, and violent, meanings allowing different stakeholders to reframe the abuse and violence that women undergoing childbirth experience as natural, expected, and accepted, reinforcing specific gender dynamics not merely in maternity care but in society overall. Finally, the framework to tackle OV within the human rights approach developed by the author is expected to be advantageous for the development of further research, programs, and policies to prevent OV in Latin America and beyond.

Resumen: El síndrome Cornelia de Lange (SCdL) es un desorden congénito del desarrollo, que cursa con dismorfia facial, retraso de crecimiento y psicomotor, reducción de las extremidades, hirsutismo y afectación variable de otros aparatos o sistemas. Tiene una prevalencia baja estimándose en 1 de cada 15000 nacidos vivos, por lo que ha sido considerado como una enfermedad rara. Se conocen cinco genes causales (NIPBL , SMC1A, SMC3, HDAC8 y RAD21) que codifican proteínas reguladoras y estructurales del complejo de cohesinas. La ausencia de diagnóstico en cerca del 30% de los pacientes y la gran variabilidad fenotípica, ha sugerido la existencia de otros genes responsables, y quizás también la implicación de otros mecanismos moleculares. El reciente descubrimiento de que NIPBL podía actuar como una proteína remodeladora de la cromatina, ha hecho proponer que el SCdL debería ser considerado más un Desorden de la Cromatina que una Cohesinopatía. El objetivo general de esta memoria ha sido establecer nuevas correlaciones genotipofenotipo dentro del espectro del Síndrome Cornelia de Lange. Para ello se han estudiado casos atípicos que, muchas veces, tenían alteraciones moleculares únicas. Todo ello ha servido para buscar una explicación a la gran heterogeneidad clínica del síndrome. Como método de trabajo se han realizado historias clínicas exhaustivas, acompañadas de exploraciones físicas detalladas y de pruebas complementarias. Asimismo, se han evaluado alteraciones moleculares como variaciones en el número de copias de genes, presencia de mosaicismo somático o coexistencia de más de un síndrome. Además, el uso de nuevas tecnologías como la secuenciación masiva o paneles específicos, ha permitido proponer aproximaciones diagnósticas efectivas y novedosas para estos pacientes. En el primer trabajo se hace una descripción clínica detallada de un paciente portador de una duplicación en el gen SMC1A. A través de estudios como el cariotipo de alta resolución, el HGC y el Western blot, se confirma que la duplicación está incluida dentro de un cromosoma marcador y que produce un aumento de expresión de la proteína. Sin embargo, a pesar de que este gen es uno de los causales del SCdL, la Gestalt del paciente lo aleja de este cuadro y su fenotipo solo ha podido ser considerado como el de una cohesinopatía sin tipificar. En el segundo trabajo, se evalúa a un sujeto con SCdL y mutación en NIPBL, que presenta uno fenotipo nuevo de reducción de extremidades, caracterizado por afectación ipsilateral derecha con agenesia de peroné e hipoplasia de tibia. El estudio por pirosecuenciación en muestras de diferente origen embrionario descarta un posible mosaicismo somático. Además, la utilización de HCG y la secuenciación masiva confirman la falta de implicación de otros genes causales. Estos hallazgos amplían nuestro conocimiento sobre los defectos de reducción que pueden acompañar al síndrome. En el tercer trabajo se describe como gracias a las nuevas técnicas de secuenciación masiva se diagnóstica un paciente con SCdL y mosaicismo somático del gen NIPBL. Inicialmente, el paciente fue estudiado mediante secuenciación Sanger con resultado negativo. Sin embargo, el hallazgo de la mutación mediante técnicas de secuenciación masiva, sirvió para reforzar la idea, de que parte de los pacientes no diagnosticados podrían padecer una mutación en estado mosaico, y de que las técnicas más adecuadas para descubrirlo, serían, combinar la secuenciación masiva con el estudio de ADN de células epiteliales de mucosa oral o fibroblastos. En el último trabajo se describe una paciente con SCdL y Síndrome de Turner en estado mosaico. Esta coincidencia y la observación de que el Turner es posterior temporalmente a la mutación de NIPBL, sirve para sugerir la hipótesis, de que las mutaciones del complejo de cohesinas podrían estar relacionadas con alteraciones en el reordenamiento cromosómico.

Resumen: El estudio indaga en las lógicas de la ciudadanía digital y la mediatización política en redes sociales digitales. Para esto, se analizan las expresiones hipermediales de ciudadanía en la red social digital Twitter que se dieron en la coyuntura del paro nacional en Colombia en noviembre de 2019. El corpus de estudio consta de 182 tweets que se abordaron metodológicamente desde la etnografía virtual y el análisis del discurso multimodal. El estudio argumenta que la frecuencia de uso de los medios, y las tecnologías que los soportan han reacomodado las fronteras entre lo público y lo privado, lo local y lo global, así como los lenguajes disponibles y posibilidades expresivas para la producción y consumo simbólico. En ese sentido, se destaca que la popularización de las redes sociales digitales ha llevado a un nuevo nivel la expresión de subjetividades, lo cual incluye la participación política.

Abstract: According to the World Bank, with the dramatic increase of the world´s population in the last decade, the generation of solid waste has reached 2.01 billion tonnes/annually (almost a kilogram per person daily). Bearing into mind that urban centres concentrate almost 70% of the world´s inhabitants and are the most technologically developed areas, the necessity to run them as Sustainable and Smart cities is vital for the planet´s health. One of the main and most threatening sources of waste are lithium-ion batteries (LIBs), due to the lack of logistics and technology to handle them properly, despite the increasing popularity of their usage. Besides mobile devices (phones and laptops), LIBs feed several public transportation networks, self-owned vehicles (cars, bicycles, and scooters) and mobility shared services. In fact, 140-million new electric vehicles are expected to replace internal combustion engines in the following decade worldwide, which will reduce the carbon footprint of cities, but can become a potential source of environmental, social and economic challenges if the 11-million metric tons of E-waste are not addressed from a Whole-Lifecycle perspective. This trend has been accelerated by the changing commuting dynamics and increasing social fragility triggered by COVID-19. Therefore, researchers, policy- and decision-makers are struggling to find environmentally safe disposal strategies to reach the balance between development and sustainability within the built environment. Taking this into account, the thesis presents an approach related to the most efficient solution of integrating a closed-loop reverse-logistics Circular Economy into the batterie´s supply chain. Throughout the document, the authors developed a feasible business model dedicated to collect and process LIBs to generate high-purity components to produce new batteries. The business conceptualization implied an extensive understanding of the stateof-the-art of the sector (SWOT and PESTLE analysis), an analytic benchmarking process, and the implementation of Industry-4.0 and Lifecycle Market Seeking. The result was a detailed business plan that maximised the organizational and financial aspects in order to optimize processes and resources, the strategic location of the pilot factory based on a Multicriteria Analysis, and a 3-levelled hierarchical organizational planning. The final part of the document deepened into the pilot phase by developing the architectural masterplan of the spokes (collection points) and hubs (factory). Furthermore, some expansion plans were discussed at a strategic level to propose future research topics.

Abstract: The environmental burdens of cement production and the construction field play a major role in the current climate crisis. Nearly 50 % of the carbon emissions are associated with the burning of limestone and cannot be avoided unless a different raw material is used. Additionally, the construction and demolition waste is an important source of debris that typically ends up in landfills, even though it has a significant potential for upgrading into recycled cement. In this research, the thermal treatment at clinkering temperatures of hydrated Ordinary Portland cement and mortars was evaluated as a source for cement production. Hydrated blends with ground granulated blast furnace slags were evaluated as well. The thermal treatment was comparatively performed in an electric furnace at 1250 °C, 1350 °C, and 1450 °C and 2.45 GHz microwave furnace at input powers of 0.5 kW, 1 kW, 1.5 kW, and 2 kW. Microwaves are a promising technology to assure greener industrial thermal processes due to lower energy consumption, high heating rates, and low processing times. The obtained materials were characterized by X-ray Fluorescence, X-ray Diffraction, Thermogravimetric Analysis, and Differential Scanning Calorimetry. Furthermore, performance characterization was done with calorimetry and compressive strength. Finally, microstructure analysis was accomplished using Scanning Electron Microscopy and Energy Dispersive X-ray analysis. Cement type CEMI 52.5R was used as a reference. In Addition, the microwave heating was simulated in COMSOL multiphysics for a better understanding of the interaction between the cement-based materials and the microwave irradiation. The geometry of the furnace’s cavity, the input power, the heating rate, and the influence of the fraction of quartz were evaluated. The experimentation proved that microwaving is a suitable technology to recover clinker phases from hydrated samples. The materials obtained were significantly similar in mineralogic composition to the reference cement. However, the presence of quartz drastically hindered the formation of the primary clinker phases while slags additions enhanced the formation of belite rather than to alite. The mechanical characterization showed that mortars produced with 20 % of replacement of the reference Ordinary Portland Cement by recycled cement obtained by microwave treatment develop a similar compressive strength as the reference cement reaching up to 50 MPa at 28 days similar to the 52.2 MPa expected for the reference. Moreover, the simulation demonstrated the capability for short-time processing of less than 5 min for nearly 15 g of sample. In contrast, the conventional treatment for 60 g of samples took beet 7 and 10 h. The model showed that the heating is highly localized. Processing at 0.5 kW and 1 kW are recommended for a more controlled process. Finally, from the thermal point of view, additions of nearly 20 % of quartz increase the heating rate and enable more homogeneous heating. The research proved the potential of microwave treatment for a more sustainable way to produce cement from hydrated samples and could be suitable for the cement fractions from construction and demolition waste.

Abstract: The dispersion of pollutants from urban sources needs to be investigated and the mechanisms which have an influence on the dispersion need to be totally understood. UAV-based systems offer great potential for mobile exploration of air pollutants in the lower atmosphere due to the high maneuverability of multi-rotor UAVs. The aim of this study was to develop a UAV platform capable of performing high-resolution three-dimensional profiling of pollutants such as particulate matter (PM), ultrafine particles (UFP), black carbon (BC), as well as meteorological parameters including temperature, relative humidity, pressure, wind speed, and wind direction. This study included a smoke test to examine the propeller-caused airflows. The results showed that the sensor air inlets and wind sensor should be located at least 90 cm above the propeller level. In addition, a field test was conducted to evaluate the stability of the UAV platform and the in-flight performance of the sensors. During the three flights carried out, the UAV platform was stable, and the data collected with the sensors were consistent. Finally, a three-day measurement campaign was conducted to examine the short-term three-dimensional variation of the traffic-related pollutant concentrations near the federal road B14. The vertical profiles showed that concentrations were higher on the ground due to the proximity to the source and that high wind speeds help the fast dispersion of the pollutants. The horizontal profiles showed that the pollutant concentrations were higher at the roadside and decreased with increasing distance from the road. In conclusion, this UAV platform represents a low-cost, practical, and reliable method to study the three-dimensional distribution of pollutants near the source. Resumen: La dispersión de contaminates originado por fuentes urbanas necesita ser investigado y los mecanismos que tienen influencia en la dispersión deben ser totalmente comprendidos. Los sistemas basados en VANT ofrecen gran potencial en la exploración de contaminantes del aire en la parte inferior de la atmósfera debido a la alta manejabilidad de los VANT multirotor. El objetivo de este estudio fue el desarrollo de una plataforma, usando un VANT, capaz de realizar perfiles tridimensionales de alta resolución de contaminantes como material particulado, particulas ultrafinas, carbón negro, así como parámetros meteorológicos incluyendo temperatura, humedad relativa, presión, velocidad y dirección del viento. Este estudio incluyó una prueba de humo para examinar los flujos de aire provocados por las hélices del VANT. Los resultados mostraron que las entradas de aire de los sensores y el sensor de viento deben ubicarse al menos 90 cm por encima del nivel de la hélice. Además, se realizó una prueba de campo para evaluar la estabilidad del VANT equipado y el desempeño de los sensores en vuelo. Durante los tres vuelos realizados, VANT se mantuvo estable y los datos recolectados con los sensores fueron consistentes. Finalmente, se llevaron a cabo mediciones durante tres días para examinar la variación tridimensional a corto plazo de las concentraciones de contaminantes relacionados con el tráfico cerca de la carretera federal B14 en Stuttgart. Los perfiles verticales mostraron que las concentraciones eran mayores en el suelo debido a la proximidad a la fuente y que las altas velocidades del viento ayudan a la rápida dispersión de los contaminantes. Los perfiles horizontales mostraron que las concentraciones de contaminantes eran más altas al costado de la carretera y disminuían al aumentar la distancia desde la carretera. En conclusión, este VANT equipado representa un método de bajo costo, práctico y confiable para estudiar la distribución tridimensional de contaminantes cerca de la fuente.

Abstract: Using Scrum as the central process framework for capstone project courses has shown to be beneficial for the learning of the students. However, these capstone project courses rarely focus on teaching students how to become a Scrum Master and do not empower them to assume the role of the Scrum Master. As a consequence, not much is known on the responsibilities that are actually assumed by those few students assuming the role of the Scrum Master in capstone project courses. Moreover, there is not much known about how these students should be taught in order to become a proficient Scrum Master. In this thesis, a case study was carried out in the Software Project 3 course held at Aalto University. The Software Project 3 course is one of the few capstone project courses that allows the students to assume the role of the Scrum Master and supports student learning through complementary teaching methods. This thesis aimed to identify the responsibilities assumed by the Scrum Master in capstone projects, and how successfully the Scrum Master contributed to the teamwork and team effectiveness of the Development Team. In addition, this study attempted to assess the contribution of complementary teaching methods, employed in the Software Project 3 course, to the learning of the students on how to become a proficient Scrum Master. This study also aimed to identify the lessons learnt by the students who assumed the role of the Scrum Master in the Software Project 3 course. In this study, the data was collected through interviews with the Scrum Masters, surveys targeted at both Scrum Masters and Developers, as well as data collected from the course content and artifacts submitted by the teams for the project reviews. They were able to successfully promote teamwork and team effectiveness within the Development Team. In addition, The results of this study show that the students, who acted as a Scrum Master, assumed all the responsibilities of the Scrum Master defined in the Scrum Guide However, in this capstone project course, these students also undertook additional responsibilities that are not explicitly mentioned in the Scrum Guide or should have been assumed by the Development Team or Product Owner. Assessment of the complementary teaching methods shows that the two-day Certified Scrum Master course and the Scrum Game, were highly valued by most of the students who felt that these two teaching methods contributed to their learning on how to become a Scrum Master. Coaching was perceived to contribute positively to the quality of the project. However, the opinion about coaching was not shared by all the students. Experience Exchange Sessions were deemed to contribute little to the learning of students. Although students faced several difficulties throughout the project that hindered the implementation of Scrum, all of them undertook the role of the Scrum Master successfully and learnt how to promote and support Scrum in their teams.

Abstract: The amygdala is a key hub for the acquisition, storage and extinction of fear memories, which are important in adaptive behaviors. The intercalated cell clusters (ITCs) are groups of inhibitory cells within the amygdaloid complex that have been recently shown to play an important role in fear-related states, especially in the context of fear extinction. Apart from the canonical view that points ITCs as feedforward and feedback inhibitors between the input and output stations towards motor centers, it has been starting to suggest that they could have diverse outputs to different regions beyond the amygdaloid complex. Although some preliminary studies have given some indications of this potential new role of the ITCs, indicating an increase in the complexity of their function in fear behavior and emotional processing, very little is still known. In this thesis, two ITC clusters –lateral and dorsomedial– are the object of the study and two main questions are addressed: (1) Do lateral and dorsomedial ITCs (latITCs and dmITCs) extend long-range projections towards the non-lemniscal (NL) thalamus and the dopaminergic (DA) midbrain? If yes, are their innervation patterns different from each other across these structures? I evaluated the outward latITCs and dmITCs projections to the NL-thalamic and DA-midbrain subnuclei, using anterograde tracing upon Cre-dependent expression of ChR2-EYFP in FoxP2-Cre transgenic mice and less extensively, using retrograde tracing to confirm the results from the anterograde tracing. With these methodological approaches I showed that latITCs and dmITCs do send long-range projections in a differential fashion towards the distinct examined subnuclei. I also showed that these projections are inhibitory (most likely GABAergic) and also, that to assess the innervation pattern, it is necessary to make either automated counting of the fibers, or measure the general innervation in terms of at least two variables–area coverage and fibers’ density– that indirectly provide a more comprehensive innervation index. Resumen: La amígdala es un centro fundamental en la adquisición, almacenamiento y extinción de memorias de miedo, que son importantes para los comportamientos adaptativos. Los grupos de células intercaladas (ITCs) se localizan del complejo de la amígdala, y la regulación inhibitoria sobre ésta, ha demostrado demostrado tener un papel importante en los estados relacionados con el miedo, especialmente en el contexto de la extinción. Aparte de la visión canónica que señala a las ITCs como inhibidores retroalimentadores y proalimentadores entre las estaciones de input y output hacia los centros motores, se ha empezado a sugerir que podrían tener diversas salidas hacia diferentes regiones más allá del complejo de la amígdala. A pesar de que algunos estudios preliminares han dado algunos indicios que soportan este nuevo rol de las ITCs, indicando un aumento de la complejidad de su función en el comportamiento del miedo y el procesamiento emocional, actualmente se conoce muy poco sobre este rol extendido. En esta tesis, dos grupos de células intercaladas diferentes -lateral y dorsomedial- son objeto de estudio y se abordan dos cuestiones principales (1) ¿Los ITCs laterales y dorsomediales (latITCs y dmITCs) extienden proyecciones extendidas hacia núcleos no lemniscales del tálamo (NL) y el cerebro medio dopaminérgico (DA)? En caso de hacerlo, ¿se presenta un patrón inervación diferencial hacia estas estructuras? Para responder esto, he evaluado las proyecciones de los grupos latITCs y dmITCs hacia múltiples subnúcleos del tálamo no lemniscal y del mesencéfalo dopaminérgico, utilizando el trazado viral anterógrado tras la expresión de ChR2-EYFP, que es dependiente del sistema Cre, en ratones transgénicos FoxP2-Cre y, de forma menos extensa, utilizando el trazado retrógrado para confirmar los resultados del trazado anterógrado. Con estos enfoques metodológicos me fue posible demostrar que los grupos de latITCs y dmITCs envían proyecciones extendidas hacia los distintos subnúcleos examinados, de forma diferencial. También demostré que estas proyecciones son inhibitorias (muy probablemente GABAérgicas) y también, que para evaluar el patrón de inervación, es necesario hacer un recuento automatizado de las fibras (por medio de un algoritmo), o medir la inervación general en términos de al menos dos variables -cobertura de área y densidad de fibras- que de forma indirecta, proporcionan un índice de inervación más completo.