Abstract: Metarhizium brunneum is a soil-borne fungal entomopathogen that can associate with plant roots. Previous studies have demonstrated that root colonization by beneficial fungi can directly affect soilborne pathogens through competition and antibiosis, and can activate a systemic response in plants, resulting in a primed state for a faster and/or stronger response to stressors. However, the mechanisms by which Metarhizium inoculation ameliorates symptoms caused by plant pathogens are not well known. This study evaluated the ability of M. brunneum to protect oilseed rape (Brassica napus L.) plants against the soil-borne pathogen Verticillium longisporum, and investigated whether the observed effects are a result of direct interaction and/or plant-mediated effects. In vitro and greenhouse experiments were conducted to measure fungal colonization of the rhizosphere and plant tissues, and targeted gene expression analysis was used to evaluate the plant response. The results show that M. brunneum delayed pathogen colonization of plant root tissues, resulting in decreased disease symptoms. Direct competition and antibiosis were found to be part of the mechanisms, as M. brunneum growth was stimulated by the pathogen and inhibited the in vitro growth of V. longisporum. Additionally, M. brunneum changed the plant response to the pathogen by locally activating key defence hormones in the salicylic acid (SA) and abscisic acid (ABA) pathways. Using a split-root setup, it was demonstrated that there is a plant-mediated effect, as improved plant growth and decreased disease symptoms were observed when M. brunneum was in the systemic compartment. Moreover, a stronger systemic induction of the gene PR1 suggested a priming effect, involving the SA pathway. Overall, this study sheds light on the mechanisms underlying the protective effects of M. brunneum against soil-borne pathogens in oilseed rape plants, highlighting the potential of this fungal entomopathogen as a biocontrol agent in sustainable agriculture Metarhizium brunneum es un hongo entomopatógeno del suelo que puede asociarse con las raíces de las plantas. Resumen: Estudios previos han demostrado que la colonización de raíces por hongos beneficiosos puede afectar a patógenos del suelo a través de la competencia, antibiosis, y activar respuesta sistémica en las plants. Sin embargo, los mecanismos por los cuales este hongo atenúa los síntomas causados por fitopatógenos no son bien conocidos. Este estudio evaluó la capacidad de M. brunneum para proteger las plantas de colza (Brassica napus L.) contra Verticillium longisporum, e investigó si los efectos observados son el resultado de una interacción directa y/o efectos mediados por la planta. Se realizaron experimentos in vitro y en invernadero para medir la colonización fúngica de la rizosfera y los tejidos de la planta, y se midió la expresión de genes específicos relacionados con la respuesta de la planta a patógenos. Los resultados muestran que M. brunneum retrasó la colonización del patógeno en las raíces de la planta, y disminuyo los síntomas de la enfermedad. La competencia directa y la antibiosis son parte de los mecanismos, ya que el crecimiento de M. brunneum fue estimulado por el patógeno e inhibió el crecimiento in vitro de V. longisporum. Además, M. brunneum cambió la respuesta de la planta hacia el patógeno al activar localmente genes de defensa mediadas por el ácido salicílico (SA) y del ácido abscísico. Mediante un experimento de raíces divididas, se demostró que existe un efecto mediado por la planta, ya que se observó un mejor crecimiento de la planta y una disminución de los síntomas de la enfermedad cuando M. brunneum estaba en el compartimento sistémico. Además, una mayor inducción sistémica del gen PR1 sugirió un efecto de ""priming"", mediado por SA.

Abstract: The use of written texts in English for oral and written communication in Spanish in higher education settings is not uncommon today. The impact that this has on the lexical level has not yet been evaluated in the specialized communicative context of Colombian university higher education. As we will see, in this context the use of terminological Anglicisms is surpassing the Spanish terms that already exist to refer to the same concepts.

Abstract: This paper proposes a pipeline for the automation of data between two realms: i) simulation, in a fully nonlinear, and time-dependent structural analysis model and, ii) concrete maturity monitoring data from the construction site. The connection enables an information construct understood for its use within the Digital Twin of the building during construction phases. The pipeline requires a comprehensive coordination between stakeholders at both the site (Construction) and the structural control office, which is challenging. The pipeline consists of a) temperature sensors, b) a mobile app connected to the sensor via Bluetooth with basic instructions for datagatherers, c) integration and interoperability of BIM, and, d) an advanced Finite Element (FE) model. By measuring the concrete temperature during many days, realistic concrete mechanical properties are inferred and infused into the FE models using adequate calibration. Two applications for the improvement of construction activities are identified. Formwork striking and tendons stressing. The paper describes the testbed of all the connections, for the construction of an in-situ casted concrete building in Barcelona, Spain.

Resumen: Uno de los mamíferos más emblemáticos de la Orinoquia colombiana es el hormiguero gigante, Myrmecophaga tridactyla, más conocido como oso palmero. Presenta adaptaciones anatómicas y comportamentales relacionadas con su alimentación especializada. Una de esas adaptaciones es el miembro torácico. En Colombia no se ha estudiado la relación entre las características morfológicas del miembro torácico y su funcionalidad. Por lo tanto, el objetivo de este estudio fue caracterizar morfológica y funcionalmente el miembro torácico de ejemplares de M. tridactyla de la Orinoquia colombiana. Se encontró que el miembro torácico se caracteriza por presentar unos músculos bien desarrollados, con aumento de áreas de inserción. Por otra parte, los huesos presentan accidentes óseos, crestas y procesos que incrementan la efectividad de los movimientos musculares. Las proporciones óseas de los huesos ulna y húmero indicaron que el miembro puede ejercer movimientos rápidos, fuertes, y de tipo excavatorio. Se evidenció que durante el forrajeo, diferentes movimientos musculares actúan entre sí para ejecutar una poderosa flexión digital, retracción del miembro y rotación de la mano. En conclusión, el miembro torácico del hormiguero gigante se encuentra adaptado morfológicamente a diferentes tipos de hábitos locomotores. Además, las características morfológicas de sus elementos óseos explican su forma de caminar en nudillos y comportamiento. El conocimiento anatómico y funcional puede servir como herramienta para prácticas médicas y manejo de ejemplares en cautiverio. También para entender el impacto que pueden llegar a tener la transformación de ecosistemas, en la fisiología y uso de hábitat en esta especie amenazada.

Abstract: Agricultural intensification is a persistent and growing threat to biodiversity worldwide. Olive groves cover extensive areasin the Mediterranean basin and play a fundamental role as refuge and wintering quarters for many bird species, but the effectsof their current intensification on bird communities remain unclear. This study aims to evaluate the response of wintering birdsto the management of olive groves with different degrees of intensification (traditional, intensive and superintensive manage-ment) in central Spain. Based on two bird censuses conducted in 25 groves in early and late winter, we examined the influenceof habitat structure and composition at different spatial scales on species richness and abundance of the entire farmland birdcommunity, and of the dietbased functional groups. Total species richness tended to decrease with intensive olive grove management, probably due to reduced habitat heterogeneity, whereas total abundance did not, indicating the capacity of intensiveand super-intensive olive groves to sustain large numbers of wintering birds. The negative effect of intensification was particu-larly evident in frugivore species richness, while frugivore abundance was positively (but marginally) associated with olive fruitavailability. Granivorous species were positively associated with lower vegetation cover, but insectivorous species did notrespond to intensification gradients. Species richness and abundance (total and by diet-based functional groups) also decreasedfrom early to late winter, which may be due to the effect of olive harvest, but also to an extreme cold event. Overall, our find-ings show how olive grove intensification and the associated management practices result in significant changes in habitatstructure and composition at different spatial scales, thus affecting wintering bird communities.

Abstract: Objective: To evaluate the effect of post-curing method, printing layer thickness, and water storage on the me chanical properties and degree of conversion of a light-curing methacrylate based resin material (IMPRIMO® LC Splint), used for the fabrication of 3D printed occlusal splints and surgical guides. Methods: 96 bar-shaped specimens were 3D printed (Asiga MAX), half of them with a layer thickness of 100 ? m (Group A), and half with 50 ? m (Group B). Each group was divided in three subgroups based on the post-curing method used: post-curing with light emitting diode (LED) and nitrogen gas; post-curing with only LED; and non- post-curing. Half of the specimens from each subgroup were water-stored for 30 days while the other half was dry-stored (n = 8). Flexural strength and flexural modulus were evaluated. Additional specimens were prepared and divided in the same way for surface hardness (n = 96), fracture toughness, and work of fracture (n = 96). Five specimens were selected from each subgroup for evaluating the degree of conversion (DC). Data were collected and statistically analyzed with 1-way, 2-way ANOVA, and Tukey post-hoc analysis (? = 0.05). Results: The 2-way ANOVA showed that the post-curing method and water storage significantly affected the investigated mechanical properties (P < 0.001). The 1-way ANOVA revealed a statistically significant difference among the tested groups on the investigated properties (P < 0.001). After water storage, the 100 ? m subgroup post-cured with only LED showed higher flexural strength (51 ± 9) than the 50 ? m and 100 ? m subgroups that were post-cured with LED in addition to nitrogen gas atmosphere (38 ± 5, 30 ± 3) (p < 0.05). The 50 ? m subgroup post-cured with only LED showed the highest significant flexural modulus values (1.7 ± 0.08) (p < 0.05). However, the 50 ? m subgroup post-cured with LED plus nitrogen showed significantly higher surface hardness values (p < 0.05) among the investigated groups. The non-post-cured subgroups showed the lowest values, which were significantly different from the other subgroups (p < 0.05). Conclusion: The post-curing method, water storage, and printing layer thickness play a role in the mechanical properties of the investigated 3D Printed occlusal splints material. The combination of heat and light within the post-curing unit can enhance the mechanical properties and degree of conversion of 3D printed occlusal splints. Flexural strength and surface hardness can increase when decreasing printing layer thickness. Resumen: Objetivo: Evaluar el efecto del método de poscurado, el espesor de la capa de impresión y el almacenamiento de agua en el tiempo. Propiedades mecánicas y grado de conversión de un material de resina fotopolimerizable a base de metacrilato (IMPRIMO® LC Splint), utilizado para la fabricación de férulas oclusales y guías quirúrgicas impresas en 3D. Métodos: Se imprimieron en 3D (Asiga MAX) 96 muestras en forma de barra, la mitad de ellas con un espesor de capa de 100 ? m. (Grupo A), y la mitad con 50 ? m (Grupo B). Cada grupo se dividió en tres subgrupos según el poscurado. método utilizado: postcurado con diodo emisor de luz (LED) y gas nitrógeno; postcurado solo con LED; y no- después de curado. La mitad de los especímenes de cada subgrupo se almacenaron en agua durante 30 días mientras que la otra mitad se almacenó en agua. almacenado en seco (n = 8). Se evaluaron la resistencia a la flexión y el módulo de flexión. Se prepararon muestras adicionales. y dividido de la misma manera para dureza de la superficie (n = 96), tenacidad a la fractura y trabajo de fractura (n = 96). Se seleccionaron cinco ejemplares de cada subgrupo para evaluar el grado de conversión (DC). Los datos fueron recopilados y analizados estadísticamente con ANOVA de 1 vía, 2 vías y análisis posthoc de Tukey (? = 0,05).

Abstract: The purpose of this in vitro study was to evaluate the mechanical properties of 3 prepolymerized polymethyl methacrylate (PMMA) resins used in the fabrication of computer- aided design and computer-aided manufacturing (CAD-CAM) milled complete dentures (CDs), as well as 2 denture base polymers used for conventionally fabricated CDs. Material and methods. Three CAD-CAM materials were evaluated: Degos Dental L-Temp, IvoBase CAD, and Zirkonzahn Temp Basic Tissue. Two materials used for conventionally manufactured dentures were also included as controls (Palapress and Paladon 65). Each material type was sectioned into bars for flexural strength, nanohardness, elastic modulus, and surface microhardness evaluation (n=8/material). Half of the specimens were stored in water for 30 days, while the other half was dry-stored. A 2-way ANOVA was conducted to detect the effect of material and storage on the evaluated properties (a =.05). Linear contrasts were conducted to compare the differences among the 3 types of CAD-CAM material and the conventional ones. Results. Material type and storage had a significant influence on the flexural strength, nanohardness, elastic modulus, and surface hardness of the materials investigated (P<.001). The post hoc Scheffé test for flexural strength revealed a nonsignificant difference in the interaction between Degos L-Temp and Paladon (P=1.000). In terms of nanohardness, no difference was found when comparing Palapress with Paladon, as well as IvoBase CAD with Zirkonzahn Temp Basic (P=1.000). A nonsignificant interaction in terms of surface hardness was also found between IvoBase CAD and Palapress (P=.575). Resumen: El propósito de este estudio in vitro fue evaluar las propiedades mecánicas de 3 resinas prepolimerizadas de polimetilmetacrilato (PMMA) utilizadas en la fabricación de computadoras Diseño asistido y fabricación asistida por ordenador (CAD-CAM) de prótesis dentales completas fresadas (CD), así como así como 2 polímeros de base para dentaduras postizas utilizados para los CD fabricados de forma convencional. Material y métodos. Se evaluaron tres materiales CAD-CAM: Degos Dental L-Temp, IvoBase CAD y Zirkonzahn Temp Basic Tissue. Dos materiales utilizados de forma convencional. Las dentaduras postizas fabricadas también se incluyeron como controles (Palapress y Paladon 65). Cada El tipo de material se seccionó en barras para determinar la resistencia a la flexión, la nanodureza, el módulo elástico, y evaluación de microdureza superficial (n=8/material). La mitad de los especímenes fueron almacenados en agua durante 30 días, mientras que la otra mitad se almacenó en seco. Se realizó un ANOVA de 2 vías para detectar el efecto del material y el almacenamiento sobre las propiedades evaluadas (a =.05). Lineal Se realizaron contrastes para comparar las diferencias entre los 3 tipos de CAD-CAM. materiales y los convencionales. Resultados. El tipo de material y el almacenamiento tuvieron una influencia significativa en la resistencia a la flexión, nanodureza, módulo elástico y dureza superficial de los materiales investigados (P<0,001). El La prueba post hoc de Scheffé para la resistencia a la flexión reveló una diferencia no significativa en la interacción entre Degos L-Temp y Paladon (P=1.000). En términos de nanodureza, no se encontraron diferencias. Encontrado al comparar Palapress con Paladon, así como IvoBase CAD con Zirkonzahn Temp Básico (P=1.000). También se encontró una interacción no significativa en términos de dureza superficial. entre IvoBase CAD y Palapress (p=0,575).

Abstract: The research aimed to determine the tensile bond strength (TBS) between polymerized intact and ground fiber-reinforced composite (FRC) surfaces. FRC prepregs (a reinforcing fiber pre-impregnated with a semi-interpenetrating polymer network (semi-IPN) resin system; everStick C&B) were divided into two groups: intact FRCs (with a highly PMMA-enriched surface) and ground FRCs (with a low PMMA gradient). Each FRC group was treated with: StickRESIN and G-Multi PRIMER. These groups were further divided into four subgroups based on the application time of the treatment agents: 0.5, 1, 2, and 5 min. Next, a resin luting cement was applied to the FRC substrates on the top of the photo polymerized treating agent. Thereafter, weight loss, surface microhardness, and TBS were evaluated. Three-factor analysis of variance (p ? 0.05) revealed significant differences in the TBS among the FRC groups. The highest TBS was recorded for the intact FRC surface treated with G-Multi PRIMER for 2 min (13.0 ± 1.2 MPa). The monomers and solvents of G-Multi PRIMER showed a time-dependent relationship between treatment time and TBS. They could diffuse into the FRC surface that has a higher PMMA gradient, further resulting in a high TBS between the FRC and resin luting cement. Resumen: La investigación tuvo como objetivo determinar la resistencia a la tracción (TBS) entre polimerizados Superficies compuestas reforzadas con fibra (FRC) intactas y rectificadas. Prepregs de FRC (una fibra de refuerzo preimpregnado con un sistema de resina de red polimérica semiinterpenetrante (semi-IPN); siempreStick C&B) se dividieron en dos grupos: FRC intactos (con una superficie altamente enriquecida en PMMA) y molidos. FRC (con un gradiente bajo de PMMA). Cada grupo de FRC fue tratado con: StickRESIN y G-Multi CEBADOR. Estos grupos se dividieron en cuatro subgrupos según el tiempo de aplicación del agentes de tratamiento: 0,5, 1, 2 y 5 min. A continuación, se aplicó un cemento de resina a los sustratos de FRC. en la parte superior del agente de tratamiento fotopolimerizado. Posteriormente, pérdida de peso, microdureza superficial, y TBS fueron evaluados. El análisis de varianza de tres factores (p ? 0,05) reveló diferencias significativas en el TBS entre los grupos del FRC. El TBS más alto se registró para la superficie intacta de FRC tratada con G-Multi PRIMER durante 2 min (13,0 ± 1,2 MPa). Los monómeros y disolventes de G-Multi PRIMER mostró una relación dependiente del tiempo entre el tiempo de tratamiento y TBS. Podrían difundirse hacia el Superficie de FRC que tiene un gradiente de PMMA más alto, lo que resulta además en un TBS alto entre el FRC y Cemento de fijación de resina.

Abstract: Statement of problem: The fracture incidence of implant-supported overdentures is more frequent in the area of attachment because of stress concentration and denture deformation in this area. How Eglass fiber reinforcement can address this problem is unclear. Purpose: The purpose of this in vitro study was to evaluate the influence of unidirectional E-glass fiber reinforcement on the mid-line denture base strains of single implant-supported overdentures. Material and methods. An experimental acrylic resin cast was constructed with a single implant placed in the mid-line area and a ball attachment screwed to the implant. Twenty-four experimental overdentures were constructed and divided into 4 groups: group AP fabricated from autopolymerizing acrylic resin without fiber reinforcement, group APF fabricated from autopolymerizing acrylic resin with unidirectional E-glass fiber reinforcement running over the residual ridge and the ball matrix, group HP fabricated from heatpolymerized acrylic resin without fiber reinforcement, and group HPF fabricated from heat-polymerized acrylic resin with unidirectional E-glass fiber reinforcement running over the residual ridge and the ball matrix. A biaxial rosette strain gauge was attached to the incisor areas of each overdenture above the attachment level (Ch1, Ch2) and to a multichannel digital strain meter. A static vertical load of 100 N was applied to the first molar area bilaterally by using a universal testing device during strain measurement procedures. The differences in the mean strain and deflection values among the investigated groups were evaluated for statistical significance using 1-way analysis of variance (ANOVA) with the Tukey post hoc multiple comparison (a =.05). Results. The type of acrylic resin did not have a statistically significant effect on the mean strain values among groups (P=.350), while the reinforcement did significantly affect them (P<.001). The interaction between reinforcement and acrylic resin was not statistically significant (P=.552). Both strain gauge channels in group APF and group HPF recorded significantly lower strain values by almost 50% than those of group AP and group HP (P<.05). Conclusions. Unidirectional E-glass fiber reinforcement placed over the residual ridge and implant attachment significantly reduced denture base strains and deformation of single implantsupported overdentures. Resumen: Declaración del problema. La incidencia de fracturas de las sobredentaduras implantosoportadas es más frecuente en el área de inserción debido a la concentración de tensiones y la deformación de la dentadura postiza en esta área. No está claro cómo el refuerzo de fibra de vidrio E puede abordar este problema. Objetivo. El propósito de este estudio in vitro fue evaluar la influencia de unidireccional Refuerzo de fibra de vidrio E en la línea media de la base de la dentadura postiza de un solo implante. Material y métodos. Se construyó un modelo experimental de resina acrílica con un solo implante. colocado en la zona de la línea media y un accesorio de bola atornillado al implante. Veinticuatro se construyeron sobredentaduras experimentales y se dividieron en 4 grupos: grupo AP fabricado de resina acrílica autopolimerizable sin refuerzo de fibra, grupo APF fabricado con resina acrílica autopolimerizable con refuerzo de fibra de vidrio E unidireccional que recorre el cresta residual y matriz de bolas, grupo HP fabricados con resina acrílica termopolimerizada sin refuerzo de fibra, y grupo HPF fabricado a partir de resina acrílica termopolimerizada con Refuerzo unidireccional de fibra de vidrio E que recorre la cresta residual y la matriz de bolas. Se adjuntó un medidor de tensión de roseta biaxial a las áreas de los incisivos de cada sobredentadura por encima del nivel de conexión (Ch1, Ch2) y a un medidor de tensión digital multicanal.

Abstract: The aim of this in vitro study was to assess the mechanical properties of five commercially available subtractive computer-aided design and computer-aided manufacturing (CAD-CAM) milled splint materials, as well as to compare them with conventional heat-polymerized and autopolymerizing resins used in the con struction of conventional splints. Material and methods: Five CAD-CAM milled (ProArt CAD Splint, Therapon Transpa, Temp Premium Flexible Transpa, Cast, and Aqua), one autopolymerizing (Palapress), and one heat-polymerized (Paladon 65) resin materials were evaluated. Flexural strength, E-modulus, Vickers hardness, fracture toughness, fracture work, water sorption, and water solubility were measured. Samples were evaluated after dry and water storage for 30 days at 37 ? C. Data were collected and statistically analyzed. Results: Under both storage circumstances, the flexural strength values of Paladon 65, Therapon Transpa, Temp Premium Flexible Transpa, and Aqua were statistically non-significant (P=0.055). The polycarbonate-based CAD-CAM material Temp Premium Flexible Transpa had the highest statistically significant values of the frac ture toughness and fracture work (P<0.001). Moreover, it exhibited the lowest percentages of water sorption and water solubility among the investigated materials (P<0.001). All of the CAD-CAM materials exhibited dry elastic moduli greater than Palapress and lower than Paladon 65. One of the CAD-CAM materials, Cast, had the highest dry Vickers hardness value, which was nonsignificant when compared to Therapon Transpa (P=0.762). Conclusion: CAD-CAM polycarbonate-based splint materials exhibit higher fracture toughness and fracture work as well as lower water sorption and solubility than polymethyl methacrylate-based ones. The mechanical characteristics of the assessed CAD-CAM milled splint materials were not typically superior to those of the conventional heat-polymerized resin. However, some of them outperformed the autopolymerizing acrylic resin in terms of flexural strength, surface microhardness, water sorption, and water solubility. Resumen: El objetivo de este estudio in vitro fue evaluar las propiedades mecánicas de cinco disponibles comercialmente materiales de férula fresados de diseño sustractivo asistido por computadora y fabricación asistida por computadora (CAD-CAM), como así como compararlos con resinas convencionales polimerizadas por calor y autopolimerizantes utilizadas en la industria. Construcción de férulas convencionales. Material y métodos: Cinco fresados CAD-CAM (ProArt CAD Splint, Therapon Transpa, Temp Premium Flexible Transpa, Cast y Aqua), una resina autopolimerizable (Palapress) y una resina termopolimerizada (Paladon 65). Se evaluaron los materiales. Resistencia a la flexión, módulo E, dureza Vickers, tenacidad a la fractura, trabajo de fractura, Se midieron la sorción de agua y la solubilidad en agua. Las muestras se evaluaron después del almacenamiento seco y en agua durante 30 días a 37 ? C. Los datos fueron recolectados y analizados estadísticamente. Resultados: En ambas circunstancias de almacenamiento, los valores de resistencia a la flexión de Paladon 65, Therapon Transpa, Temp Premium Flexible Transpa y Aqua no fueron estadísticamente significativos (P = 0,055). La base de policarbonato El material CAD-CAM Temp Premium Flexible Transpa tuvo los valores estadísticamente significativos más altos de fractura tenacidad de la estructura y trabajo de fractura (P <0,001). Además, exhibió los porcentajes más bajos de sorción de agua y solubilidad en agua entre los materiales investigados (P<0,001). Todos los materiales CAD-CAM mostraron elasticidad seca. módulos mayores que Palapress e inferiores que Paladon 65. Uno de los materiales CAD-CAM, Cast, tuvo el mayor valor de dureza Vickers seco, que no fue significativo en comparación con Therapon Transpa (P = 0,762).